Coelenterazine 400A is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc).It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.
Product Specifications:Coelenterazine 400 a2,8-Dibenzyl-6-phenyl-imidazo[1,2a]pyrazin-3-(7H)-one DeepBlueCTM
Formula: C26H21N3O
MW: 391.48 g/mol
Purity: >95% by TLC
Color: Yellow to orange powder
Storage/Handling: Store at -20°C, and protect from light. Keep under inert gas.Soluble in methanol or ethanol (NOT in DMSO). Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that willspontaneously decompose even at low temperatures.
PubChem CID:135750016
