5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeastmolecular genetics. The toxicity of 5-FOA to yeast cells with afunctioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMPdecarboxylase), makes it especially useful for the selection andidentification of mutant yeast strains.5-FOA is most often appliedwith Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli(pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for theconstruction of activation domain hybrid libraries to identifyprotein-protein interactions.
Product Specifications:5-Fluoroorotic acid monohydrate (5-FOA)5-Fluorouracil-4-carboxylic acid
MOLECULAR BIOLOGY GRADE
Formula: C5H3FN2O4 • H2O
MW: 192.10 g/mol
White to slightly off-white crystalline powder
Storage/Handling: Store at -20°C. Protect from light.
PubChem Chemical ID: 16212749
