Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue (see Figure 2).
Goof-Proof™ allows you to see quickly and easily whether you forgot to add the master mix or the template to any of your reactions, preventing pipetting mistakes and wasted time, reagents and money. The 2X Goof-Proof™ Master Mix can also be used without the Template Buffer, if desired.
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| Figure1: 1x Goof-Proof™Master Mix | Figure 2: 1x Goof-Proof™ Master Mix after adding 1X Goof-Proof™ Template Buffer |
Kit components
- Goof-Proof™ 2X Master Mix -The 2X Master Mix contains GoldBio HotStart Taq DNA Polymerase, dNTPs and a low concentration of an inert blue dye. The dye helps the user distinguish between empty wells and wells containing master mix.
- Goof-Proof™ Template Buffer -40X Template Buffer contains a high concentration of inert blue dye. Adding the template buffer to the reaction mix makes it "Goof-Proof" to track which wells have had template added to the PCR reactions. Template Buffer is not required for the Master Mix to function, but is designed to help alleviate mistakes with template addition.
- ROX Reference Dye -Proof™ qPCR Master Mix with ROX includes a vial of ROX reference dye. ROX is necessary for accurate Ct determination from well to well with certain instruments (such as some ABI and Stratagene PCR instruments, refer to your instrument instructions to see if ROX is required). ROX may add noise to melt curve analysis, which could be mistaken for real peaks. In the case of unexpected peaks, un-check “ROX” in the “Passive Reference Dye” box in the system software to prevent data collection from the ROX fluorescence channel and re-analyze the data.
Note: We also offer Goof-Proof™ qPCR Master Mix (Catalog # G-700) without ROX for instruments that do not require the ROX reference dye.
Storage/Handling: Store the kit at -20°C.
Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.
