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主营:生物发光法,活体成像技术,萤火虫荧光素酶,海肾荧光素酶
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当前位置: 首页 > 产品中心 > > GoldBio/AGL-1 Agrobacterium Chemically Competent Cells/15 x 50 μL/CC-106-15x50
商品详细GoldBio/AGL-1 Agrobacterium Chemically Competent Cells/15 x 50 μL/CC-106-15x50
GoldBio/AGL-1 Agrobacterium Chemically Competent Cells/15 x 50 μL/CC-106-15x50
GoldBio/AGL-1 Agrobacterium Chemically Competent Cells/15 x 50 μL/CC-106-15x50
商品编号: CC-106-15x50
品牌: GoldBiotechnology
市场价: ¥8980.00
美元价: 4939.00
产地: 美国(厂家直采)
公司:
产品分类: 其他
公司分类:
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Table 1: Antibiotic disc sensitivity for GoldBio’s Agrobacterium strains (using standard BD antibiotic discs)

Antibiotic Selection

Amp

Carb

Chlor

Gent

Kan

Rif

Spect

Strep

Tet

100 µg/ml

100 µg/ml

30 µg/ml

100 µg/ml

30 µg/ml

50 µg/ml

25 µg/ml

50 µg/ml

50 µg/ml

50 µg/ml

GV3101

I

R

R

PR

R

S

R

S

R

S

EHA 105

R

R/S

R

n/a

R/S

S

R

S

R

S

LBA 4404

S

S

S

n/a

S

S

R

S

R

S

AGL-1

R

R

R

n/a

R/S

S

R

S

R

S

C58C1

R

R

R

n/a

R/S

S

R

S

R

S

S = SensitiveR = ResistantR/S =intermediate zones using standard discs.I =growth in inhibitory zone with standard disc. “Opaque”, not clear zone of inhibition.

Product SpecificationsCompetent cell type: Chemical CompetentSpecies: A. tumefaciensStrain: AGL-1Transformation efficiency: ≥4 x 104 cfu/µg pCAMBIA1391z DNABlue/white screening: No

Storage/Handling: This product may be shipped on dry ice. GV3101 Agrobacterium chemically competent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

Reagents Needed for One Reaction
  • AGL-1Chemically Competent Agrobacterium: 50µl
  • DNA (pCAMBIA1391z, 10 ng/µl): 5 µl
  • Recovery medium: 1 ml

Quality ControlTransformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ≥4 x 104 CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

  • Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
  • Thaw competent cells on ice and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Calculation of Transformation EfficiencyTransformation Efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1 µg of plasmid into a given volume of competent cells.

  • TE = Colonies/µg/Dilution
    • Colonies = the number of colonies counted
    • µg = amount of DNA transformed in µg
    • Dilution = total dilution of the DNA before plating
  • Example: Transform 1 µl of (10 pg/µl) control plasmid into 25 µl of cells, add 975 µl of Recovery Medium. Dilute 10 µl of this in 990 µl of Recovery Medium and plate 50 µl. Count the colonies on the plate the next day. If you count 250 colonies, the TE is calculated as follows:Colonies = 250µg of DNA = 0.00001 Dilution = 10/1000 x 50/1000 = 0.0005 TE = 250/0.00001/0.0005 = 5.0 × 1010
品牌介绍
D-荧光素钾盐/钠盐体内使用手册--Goldbio介绍   荧光素是一种常用的荧光素酶表达的活体成像生物发光报告子。以ATP和Mg2+作为辅因子,有氧条件下荧火虫荧光素酶与水溶性底物反应发出一种特有的黄绿色发射光,在37℃活体内转换为红光。该反应通过消耗ATP来指示是否存在能量或生命的存在。   D-荧光素在生物技术领域是一种常用的试剂,尤其在活体成像方面。荧光素酶被接种在研究动物中比如大鼠,小鼠,用来标记肿瘤细胞,干细胞或传染病。通过生物荧光成像这个模式系统中D-荧光素的注射可以用来实时,非侵入性监测疾病的进程或药物效率。   产品具体信息 D-荧光素钾盐 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt KC11H7N2O3S2 分子量:318.42 g/mol D-荧光素钠盐 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt NaC11H7N2O3S2.H2O 分子量:320.32 g/mol 储存:-20℃避光保存。