4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
GoldBiotechnology(优势品牌)
主营:生物发光法,活体成像技术,萤火虫荧光素酶,海肾荧光素酶
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Other_buffers > 金生物/细菌细胞裂解缓冲液/500 mL/GB-177-500
商品详细金生物/细菌细胞裂解缓冲液/500 mL/GB-177-500
金生物/细菌细胞裂解缓冲液/500 mL/GB-177-500
金生物/细菌细胞裂解缓冲液/500 mL/GB-177-500
商品编号: GB-177-500
品牌: GoldBiotechnology
市场价: ¥2700.00
美元价: 1620.00
产地: 美国(厂家直采)
公司:
产品分类: 其它缓冲液
公司分类: Other_buffers
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Bacterial Cell Lysis Buffer is based on organic buffering agents andutilizes a mild non-ionic detergent and a proprietary combination ofvarious salts and agents to enhance extraction and stability ofproteins. Depending on the application, additional agents such asreducing agents chelating agent, and protease inhibitors may be addedinto Bacterial Cell Lysis Buffer. This reagent has been tested for usewith several widely used bacteria including E. coli strains. Bacterial Cell Lysis Buffer eliminates the need for laborious mechanical lysis of bacterial cells and removal of DNA/RNA with nuclease treatments. The proprietary combination of this reagent provides a simple and versatile method of bacterial protein extraction and isolation of inclusion bodies.

The Bacterial Cell Lysis Kit (GB-176; 100, 250 or 500 preps) includes a separate tube of modified lysozyme preparation that contains nucleases and results in optimal lysis and minimal contamination. This kit contains all of the reagents needed to perform protein extraction.For extracting soluble proteins, one prep is suitable for extracting approximately 300 mg wet cell pellets for every 1 ml Bacterial Cell Lysis Buffer. When the kits are used for extracting soluble proteins from spheroplasts, one prep is suitable for extracting approximately 90 mg wet cell pellets for every 40 μl Bacterial Suspension Buffer. Bacterial Lysis Buffer is also available separately for further downstream applications.

Storage/Handling: Store at 4°C. Do NOT freeze. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

品牌介绍
D-荧光素钾盐/钠盐体内使用手册--Goldbio介绍   荧光素是一种常用的荧光素酶表达的活体成像生物发光报告子。以ATP和Mg2+作为辅因子,有氧条件下荧火虫荧光素酶与水溶性底物反应发出一种特有的黄绿色发射光,在37℃活体内转换为红光。该反应通过消耗ATP来指示是否存在能量或生命的存在。   D-荧光素在生物技术领域是一种常用的试剂,尤其在活体成像方面。荧光素酶被接种在研究动物中比如大鼠,小鼠,用来标记肿瘤细胞,干细胞或传染病。通过生物荧光成像这个模式系统中D-荧光素的注射可以用来实时,非侵入性监测疾病的进程或药物效率。   产品具体信息 D-荧光素钾盐 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt KC11H7N2O3S2 分子量:318.42 g/mol D-荧光素钠盐 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt NaC11H7N2O3S2.H2O 分子量:320.32 g/mol 储存:-20℃避光保存。