Firefly Luciferase Assays are one of the leading reporter assays in the world in the measurement of gene function and gene regulation as well as being widely used in pharmaceutical screening. The luciferase assays are sensitive and convenient due to the absence of endogenous luciferase activity in most cell types and tissue. Firefly luciferase is a monomeric 62 kDa protein typically isolated from the firefly, Photinus pyralis, which catalyzes the ATP-dependent D-luciferin in the presence of oxygen and Mg2+ to oxyluciferin producing a yellow to greenish light (~560 nm).
GoldBio’s Illumination™ Lyophilized Firefly Luciferase Enhanced Assay Kit is specifically designed for the simple and efficient quantification of firefly luciferase reporter enzyme activity from cultured cells with the highest degree of sensitivity, reliability and linearity. In addition, the Firefly Luciferase Assay Buffer is lyophilized for more convenient shipping and storage.
This kit is a flash-type luminescent assay, and requires measurement immediately after adding the substrate to the sample.Our Enhanced Assay Kit includes our improved 5X Luciferase Lysis Buffer which provides a better working solution stability compared to other firefly assay kits.
One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Luciferase Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Luciferase Lysis Buffer (GoldBio Catalog # L-740) can be purchased separately.
Kit components
- 5X Luciferase Lysis Buffer
- Firefly Luciferase Assay Buffer (lyophilized)
- GoldBio D-Luciferin
Storage/Handling: Store the kit at -20°C (Reconstituted Assay Buffer should be stored at -80°C).
Product may be shipped on room temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.
