Firefly and Renilla Luciferase Assays are two of the leading reporter assays in the measurement of gene function and gene regulation both in vitro as well as in vivo. The luciferase assays are sensitive and convenient due to the absence of endogenous luciferase activity in most cell types and tissue. Firefly luciferase is a monomeric 62 kDa protein typically isolated from the firefly, Photinus pyralis, which catalyzes the ATP-dependent D-luciferin in the presence of oxygen and Mg2+ to oxyluciferin producing a yellow to greenish light (~560 nm). Renilla luciferase is a monomeric 36 kDa protein isolated from the sea pansy Renilla reniformis that catalyzes coelenterazine with oxygen to produce a blue light (480 nm).
GoldBio’s Illumination™ Firefly & Renilla Luciferase Enhanced Assay Kit is a combined luciferase assay allowing the sequential measurement of Firefly and Renilla luciferase activity in the same sample while still retaining the highest degree of sensitivity, reliability and linearity that you want in your assay. Firefly activity is measured first, then the Renilla Luciferase Assay Buffer is added to the sample to simultaneously extinguish all firefly luciferase activity to the level of untransformed cells and begins the Renilla luciferase activity. This great luciferase combo allows the sequential measurement of both luciferase assays in the same sample without additional hassle.
This kit is a flash-type luminescent assay, and requires measurement immediately after adding the detection reagents to the sample. The Enhanced Assay Kit includes our water soluble coelenterazine that is easier to use and store than other conventional coelenterazine products.
One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well.
Kit components
- 1X Passive Lysis Buffer
- Firefly Luciferase Assay Buffer
- Renilla Luciferase Assay Buffer
- GoldBio D-Luciferin
- GoldBio Enhanced Coelenterazine
Storage/Handling: Store the kit at -80°C (1X Passive Lysis Buffer, Luciferin and Enhanced Coelenterazine may be stored separately at -20°C).
Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.
